dermal fibroblast hs68 cell line  (ATCC)

Journal: Antioxidants

Article Title: 10(E)-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF/Tyrosinase Axis

doi: 10.3390/antiox13121547

Figure Lengend Snippet: Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) HS68, and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.

Article Snippet: The B16F10 murine melanoma cell line and Hs68 human dermal fibroblast cell line were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activity Assay, Control, Positive Control

Journal: Molecular Medicine Reports

Article Title: Improvement of wound healing by capsaicin through suppression of the inflammatory response and amelioration of the repair process

doi: 10.3892/mmr.2023.13042

Figure Lengend Snippet: Changes in Hs68 cell characteristics in the presence of CAP. Hs68 cell characteristics were examined following treatment of cells with different CAP concentrations (A) Cell viability, (B) migration (cells were also treated with MMC and TNF-α) and (C) percentage closure based on the cell migration assay were determined. (D) Relative mRNA levels of IL-6 in Hs68 cells treated with TNF-α and CAP. Scale bar, 500 µm. **P<0.01, ****P<0.0001. CAP, capsaicin; IL-6, interleukin 6; MMC, mitomycin C; TNF-α, tumor necrosis factor-α.

Article Snippet: The human diploid dermal fibroblast Hs68 cell line (passage 19), which is frequently used in dermal research and has the characteristics of primary dermal fibroblasts , was purchased from the Bioresource Collection and Research Center in the Food Industry Research and Development Institute (Hsinchu, Taiwan).

Techniques: Migration, Cell Migration Assay